Broadside is an image processing pipeline used by the Laboratory for Bio-Micro Devices @ Brigham & Women's Hospital in Boston, MA. It consists of the following pre-processing steps:
- pre-process
- make illumination profiles using pybasic
- make unmixing cache
- make corrected stacks
- register and stitch using ASHLAR
- annotate, and view unmixing results
- post-process
- nucleus and cell segmentation
- quantification and spillover correction